18 Nov Promega Corporation and SwitchGear Genomics announce a novel method for characterizing DNA-protein interactions
Madison>b> – Promega Corporation and SwitchGear Genomics announce a novel method for characterizing DNA-protein interactions. Results of a study using the HaloCHIP™ System and high-throughput promoter assays demonstrate the ability to correlate DNA binding events to transcriptional activation or repression for the CREB transcription factor. The findings appear in the article “A functional analysis of the CREB signaling pathway using HaloCHIP-chip and high throughput reporter assays”, recently published in BMC Genomics online.
This combination of technologies enables discovery of genome-wide DNA binding sites for transcription factors and the functional activities of the promoter sequences bound by those transcription factors. “This comprehensive approach enables the comparison between binding events and transcriptional activity,” said Nathan Trinklein, Ph.D., co-founder and CEO of SwitchGear Genomics. “Characterizing the functional consequences of binding events is essential to more fully understanding the control of gene expression.”
HaloCHIP, which uses Promega HaloTag® Technology, eliminates the need for antibodies to capture protein:DNA complexes, thereby avoiding one of the major challenges of chromatin immunoprecipitation (ChIP) techniques.
“HaloCHIP allows researchers to more easily and efficiently capture genome-wide protein-DNA complexes starting with a much smaller number of cells,” stated Danette Hartzell, Senior Research Scientist at Promega Corporation. “This antibody-free method for revealing DNA binding events in combination with functional reporter assays can significantly advance gene regulation studies.”